1. The preparation of material and bagging: formulas of substrate:
A. Broad-leaved tree sawdust 73%, wheat skin 10%, corncob powder 15%, sugar 0.8%, gypsum 1.1% and superphosphate 0.1%
B. Broad-leaved tree sawdust 38%, cottonseed hull 38%, wheat skin 7%, corncob powder 15%, sugar 1% and gypsum 1%.
C. Sawdust 70%, wheat skin 7%, corncob powder 13%, sugar 1%, gypsum 1% and fine soil 18%.
People could firstly mix dry materials evenly, dissolve sugar into the water, then put into all materials and mix well. People should control the water content of material in between 63% and 65%, adjust the PH Value in between 5.5 and 6, conduct bagging with plastic bags that is in the size of 17cm*33cm*0.0045cm. People could conduct bagging and tight pressing simultaneously. Moderate tightness should be mastered and the wet weight of 700g in each bag should be kept. People could use sponge tents and book sewer to seal.
2. Sterilization and inoculation: sterilization should be done immediately after bagging. People could sterilize under atmospheric pressure for 8 to 10 hours or conduct autoclaved sterilization for 1.5 to 2 hours. After sterilizing, people could open the door of stove after cooling to below 50 degrees, then move out fungus sacks and put into clean basin to cool. Inoculation can be done when the temperature of fungus sack is under 30 degrees. People could conduct lucifugal cultivation in clean, dry and sterilized cultivation room. Aseptic technique should be adhered to and 15g to 20g of inoculation amount in each bag should be controlled.
3. The cultivation of spawns: when conducting lucifugal cultivation, people could keep the temperature of material in between 22 and 28 degrees, keep relative air humidity in below 70%, ventilate in regular intervals and keep fresh air. In rainy day, people could put quick lime or charcoal to make moisture absorption. When finding the pollution of sundry fungus, people could timely conduct isolation processing. 25 to 30 days after inoculating, spawns will become fully grown and spread on the surface of substrate in suitable condition. On that occasion, people could give 100 to 200 lux of scattered light, lower 2 or 3 degrees of room temperature and promote spawns to twist into primordiums.
Fruiting management: when finding that primordiums have formed, it is best to control the temperature in between 16 and 20 degrees, spray water frequently to adjust relative air humidity in between 85% and 95%, enhance illumination intensity in between 200 and 500 lux (with sufficient scattered light) and increase ventilation. In general, when finding primordiums, caps will become eighth-developed and form tubes after 12 to 15 days of cultivation, which means that sporocarps have matured. People should pick mushroom bodies timely. Grifola frondosa is fragile in texture and easy to be broken. People should handle gently. Organic Grifola frondosa can be sold in fresh, dried and salted products. After picking, people could clean the surface of material, stop spraying water and cultivate myceliums for several days, then moderately moisturize and conduct topdressing to induce the next flush of mushroom. In general, 2 to 3 flushes of mushroom can be picked and the biological efficiency is in about 45%.
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