1. Strain selection: there are various kinds of Shiitake, common variety is low temperature type. For this kind of Shiitake, the most suitable environment during spawn running is from 24 to 27 degrees, the suitable water content of compost is about 60%, the suitable condition is dark or with low light. The most suitable environmental condition during fruiting period is from 8 to 16 degrees, has fresh air and wet climate. When choosing strains, people should choose those with no sundry fungi and pests, with pure character and robust growth trend.
2. Preparation of compost: the ingredient of compost includes broad-leaved tree sawdust, bran or rice bran, sugar, gypsum powder, urea and superphosphate. Broad-leaved tree is the main carbon source material, bran or rice bran are main nitrogen sources, people could choose fresh bran and rice bran, sugar is a quick action carbon source that can be absorbed and utilized in early growth period of spawn, both white sugar and brown sugar can be used. Gypsum powder can not only provide calcium, but also can slow down the acidification process of compost. Urea and superphosphate can provide nitrogen, phosphorus and calcium for nitrogen fertilizer and phosphatic fertilizer. The formula of compost is:
A: Broad-leaved tree sawdust 77%, bran or rice bran 20%, sugar 1.2%, gypsum powder 1.8%, urea and superphosphate.
B: Broad-leaved tree sawdust 78%, bran or rice bran 16%, sugar 1.2%, corn powder 2%, gypsum 2%, superphosphate 0.5%, urea 0.3%, sugar 1.2%. People could firstly dissolve urea, superphosphate and sugar in water, then mix with other powder materials, water-adding and mixing should be done at the same time, repeat for many times and adjust water content in 60%.
3. Sterilization: people could cut low pressure polyethylene tube film of 15cm in fold diameter mm and 0.04mm in depth into tube material of 55cm. Seal one side and put into plastic tube bags, each bag weights about 0.9Kg (dry material). After tying, people could put into atmosperic pressure steam sterilizer. Leave certain space in inner of sterilizer to promote even distribution of steam and improve the effect of sterilizing. People could warm up in high temperature fire, when the inner temperature reaches to 100 degrees, people could turn to gentle heat, continue for 10 to 12 hours and stop heating. Discharge can be done when the temperature is cooled to about 70 degrees.
4. Inoculation: when the temperature of compost tube is cooled under 28 degrees, people could firstly sterilize with 0.25% of bromogeramine solution, then move to inoculation box, add 10ml of formalin in each cubic metre and mix with 5g of potassium permanganate, close glass windows, operating hole and fumigate for 30 minutes, by using this way, people could kill microbe cell protein. After that, inoculation staffs should wash hands with 75% of ethyl alcohol, stretch into inoculation box and conduct punching and inoculation simultaneously, adhesive plaster should be taped at the same time. The process needs quick action and short time, the size of adhesive plaster could be 3.5cm*3.5cm.
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