1. Do a great job in sanitation: normal health service management system should be given to staffs on strain-making and operation, people should constantly clean pollutant, add drugs to sterilize. Mushroom house, tools and all equipment in cultivation room should be sterilized and washed. Some points on shoe sole and cloth should not be ignored.
2. Choose ways on sterilization: people can give priority to high-pressure steam sterilization, atmospheric intermittent sterilization, pasteurization and ultraviolet lamp sterilization in physical sterilization, give priority to alkaline, phenols drugs in chemical sterilization. People should avoid heavy metal salt sanitizers.
3. Good disposal on raw material: damp, mould, pests should be avoided in compost of Button mushroom, people should place plastic film or felt at the bottom to guard against damp, cover the top to prevent rain or sundries. Chicken manure, cow dung, rice bran and wheat bran are easy to breed mildew and should be kept airtightly. Compost should be went through high temperature disinfection. People should insist on doing well in secondary fermentation. Materials for earthing up should be sterilized by Formalin or steam.
4. Adjust water volume and PH Value: water volume should be proper, PH Value should be suitable. People should check and adjust all times, keep good ventilation and keep relative air humidity under 95%.
5. Keep substrate properly: people should protect bottles and sterilized substrate properly to prevent secondary pollution, cover gauze or thin film. Dust pollution should be prevented as well.
6. Strictly perform operation procedures: inoculation work has high technology and should be patiently and carefully treated. People should wash cleanly and fumigate thoroughly on all tools and vessels, ethyl alcohol also can be utilized in this kind of work.
7. Adopt artificial physical methods method to prevent disease and pests. For example, black light lamp can be used to trap and kill mosquitoes and flies, when finding rotten part, people should remove timely and sterilize with lime water. People should also improve circumstances, adjust temperature (to prevent Diehliomycesmicropours), humidity (to prevent Neurospora) and PH Value (to prevent Mucor).